MOMENTAN AUSVERKAUFT
Über dieses Produkt
Product Identifiers
PublisherSpringer New York
ISBN-101493971271
ISBN-139781493971275
eBay Product ID (ePID)237897136
Product Key Features
Number of PagesXi, 256 Pages
LanguageEnglish
Publication NameGenome Editing in Animals : Methods and Protocols
Publication Year2017
SubjectLife Sciences / Genetics & Genomics, Genetics
TypeTextbook
AuthorIzuho Hatada
Subject AreaScience, Medical
SeriesMethods in Molecular Biology Ser.
FormatHardcover
Dimensions
Item Weight229.8 Oz
Item Length10 in
Item Width7 in
Additional Product Features
LCCN2017-943506
Dewey Edition23
Series Volume Number1630
Number of Volumes1 vol.
IllustratedYes
Dewey Decimal616.02/7
Table Of ContentConstruction and Evaluation of Zinc Finger Nucleases.- Current Overview of TALEN Construction Systems.- CRISPR/Cas9.- Computational Prediction of CRISPR/Cas9 Target Sites Reveals Potential Off-Target Risks in Human and Mouse.- Genome Editing of Mouse by Cytoplasmic Injection.- Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing sgRNA/Cas9 Expressing Plasmids.- Genome Editing in Mouse and Rat by Electroporation.- Generation of Knock-in Mouse by Genome Editing.- Genome Editing of Rat.- Gene Targeting in Rabbits: Single-step Generation of Knockout Rabbits by Microinjection of CRISPR/Cas9 Plasmids.- Genome Editing of Pig.- Genome Editing of Monkey.- Genome Editing Mediated by Primordial Germ Cell in Chicken.- CRISPR/Cas9-mediated Targeted Knock-in of Exogenous Reporter Genes in Zebrafish.- Genome Editing of Medaka.- A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders using the CRISPR-Cas System.- Genome Editing of Silkworms.- Genome Editing in the Cricket, Gryllus bimaculatus.- Genome Editing of the Ascidian Ciona intestinalis with TALE Nuclease.- Genome editing of C. elegans.
SynopsisThis volume details protocols that can be used for generation of knockout animals. Chapters guide the reader through basic protocols for three genome editing technologies, target design tools, and specific protocols for each animal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Genome Editing in Animals: Methods and Protocols aims to ensure successful results in the further study of this vital field.
LC Classification NumberRB155-155.8
