Grade ToCollege Graduate Student
Table Of ContentLaboratory Overview Introduction Laboratory Safety, Equipment, and Materials Tools of the Laboratory: Basic Techniques 1 Use of the Microscope 2 Aseptic Techniques 3 Isolation Streak Plate Eukaryotic Cells and Microorganisms 4 Microbial Phototrophs: Algae and Cyanobacteria 5 Protozoa 6 Fungi 7 Bacterial Motility: Hanging Drop Method Methods for Microscopic Analysis: Staining 8 Simple Staining and Smear Preparation 9 Negative Stain 10 Capsule Stain 11 Gram Stain 12 Acid-Fast Stain (Ziehl-Neelsen and Kinyoun Methods) 13 Endospore Stain (Schaeffer-Fulton Method) Methods for Culturing and Diagnosing Microbes: Media 14 Blood Agar 15 Mannitol Salt Agar 16 Eosin Methylene Blue Agar 17 MacConkey Agar Microbial Nutrition, Ecology, and Growth 18 Nutrients and Growth: Chemical Content of Media 19 Osmotic Pressure and Growth 20 Oxygen and Growth: Aerotolerance 21 Oxygen and Growth: Catalase 22 Oxygen and Growth: Oxidase Food and Water Safety 23 Plate Count Treatment and Control of Microbial Growth 24 Killing by Ultraviolet Light 25 Antimicrobial Drug Susceptibility Testing (Kirby-Bauer Method) Microbial Genetics and Genetic Engineering 26 ELISA and Using Nucleotide BLAST (Dry Lab) 27 Analysis of DNA: Electrophoresis 28 Gene Transfer: Transformation Infectious Diseases 29 Parasitic Protozoa 30 Parasitic Worms 31 Transmission: Vectors of Disease 32 Transmission: Epidemiology Activity 33 Vaccination and Herd Immunity Diagnosing Infections 34 Gel Immunoprecipitation (Immunodiffusion) 35 Passive (Indirect) Agglutination 36 Rapid Immunoassays 37 Hemagglutinin Inhibition Assay 38 EnteroPluri-Test Environmental Microbiology 39 Soil Microbiology 40 Nitrate Reduction 41 Viral Titration Hydrolytic Enzymes and Disease Mechanisms 42 DNase Test 43 Gelatin Hydrolysis 44 Starch Hydrolysis 45 Casein Hydrolysis 46 Snyder Agar Biochemical Testing and Diagnosis of Disease 47 Phenol Red Sugar Broth 48 Triple-Sugar Iron Agar 49 Litmus Milk 50 IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate) Reactions 51 Urease Test Putting It All Together 52 Identification of Unknown Bacteria
SynopsisLaboratory Manual for Microbiology Fundamentals: A Clinical Approach is a compilation of decades of experience between the two authors, Steven Obenauf and Susan Finazzo. Their approach will add to your own cadre of techniques for effective teaching, and help your students avoid many of the common pitfalls encountered in the lab. This lab is very visually informative, and has a conversational approach which will entice student interest and facilitate learning and success in your course.