MOMENTAN AUSVERKAUFT
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Product Identifiers
PublisherOxford University Press, Incorporated
ISBN-100195089561
ISBN-139780195089561
eBay Product ID (ePID)496974
Product Key Features
Number of Pages336 Pages
LanguageEnglish
Publication NamePlant Microtechnique and Microscopy
Publication Year1999
SubjectLife Sciences / Botany, Life Sciences / Taxonomy
TypeTextbook
AuthorSteven E. Ruzin
Subject AreaScience
FormatTrade Paperback
Dimensions
Item Height0.7 in
Item Weight28.2 Oz
Item Length8.4 in
Item Width10.9 in
Additional Product Features
Intended AudienceCollege Audience
LCCN98-040910
Dewey Edition21
Reviews"Extremely well done. I cannot imagine a better combination of classical and modern techniques. It is comprehensive and should become mandatory for graduate students in microscopy."--Frank Ewers, Michigan State University, "I will value this as a reference. It is full of useful information and is organized and presented clearly."--Lee Anne Chaney, Whitworth College, "Extremely well done. I cannot imagine a better combination of classical and modern techniques. It is comprehensive and should become mandatory for graduate students in microscopy."--Frank Ewers, Michigan State University"I will value this as a reference. It is full of useful information and is organized and presented clearly."--Lee Anne Chaney, Whitworth College"Excellent resource and text for students who will be using microtechniques and microscopy. Well written and illustrated. Should be in everyone's laboratory and in the library."--David R. Dobbins, Millersville University of Pennsylvania, "Excellent resource and text for students who will be using microtechniques and microscopy. Well written and illustrated. Should be in everyone's laboratory and in the library."--David R. Dobbins, Millersville University of Pennsylvania, I am grateful to Steve Ruzin for assembling this incredibly useful collection of information in such a convenient and handy form., "Excellent resource and text for students who will be usingmicrotechniques and microscopy. Well written and illustrated. Should be ineveryone's laboratory and in the library."--David R. Dobbins, MillersvilleUniversity of Pennsylvania, "I will value this as a reference. It is full of useful information and isorganized and presented clearly."--Lee Anne Chaney, Whitworth College
IllustratedYes
Dewey Decimal580/.28/27
Table Of ContentINTRODUCTIONABBREVIATIONS1. QUICK STARTParaffin embedding--Histology or in situ hybridizationParaffin embedding--Microwave methodGlycol methacrylate embedding--HistologyButyl/methyl methacrylate embedding--in situ hybridization or immunolocalizationSteedman's wax embedding--ImmunolocalizationChromosome squashes--Maize meiocytesHematoxylin staining--Microwave techniqueAdjusting Kohler illumination2. MICROSCOPYMagnifiers and microscopesCompound microscopesPrinciples of specimen illuminationImparting contrast using inherent differences in index of refraction within the specimenConfocal and wide-field deconvolution microscopyObtaining microscopic measurements3. CHEMICAL FIXATIONTypes of chemical fixativesThe quality of fixationTissue storageThe mechanics of fixationCoagulating fixativesNoncoagulating fixativesRecommendations for fixing plant tissues4. TISSUE DEHYDRATIONGeneral protocolDehydration using a graded solvent series--Ethanol, acetoneRapid Dehydration5. INFILTRATING AND EMBEDDING TISSUESParaffin infiltrating and embeddingPolyethylene glycol and derivatives--Infiltration and embeddingPlastic infiltrating and embedding6. SECTIONING AND MOUNTINGGeneral stepsMounting blocks to microtome stubsFactors influencing the quality of sectioningThe microtome knifeSetting up the microtomeSectioning methodsMounting sections to glass slides7. STAININGChemical classification of dyesDye chemistryMicrotechnique stainsGeneral staining proceduresStaining equipmentSelected staining methods for plant microtechniqueStaining epoxy-embedded tissuesReactive dyesCytogenetic techniques with squashed materialCytogenetic stains for sectioned materialStaining pollen tubesMounting the coverslip8. ALTERNATE METHODS OF MICROTOMYFree-hand sectionsVibratomeFreezing microtome (cryotome, cryostat)Sliding microtome9. SPECIAL METHODSTissue clearingMacerating woody tissuesMacerating gymnosperm leaves for epidermal peelsMacerating nonwoody tissuesSectioning carbonized wood samplesMicroincinerationBleaching tissuesPreserving color in whole mounted specimensRepairing broken microscope slides10. MICROTECHNIQUE NOTES AND PROBLEM SOLVINGTissue collectionFixationFixatives for algaeTanninsHard materialsSliding microtomeClearingDehydration and infiltrationEmbeddingMicrotomyFree hand sectionsStaining11. HISTOCHEMISTRY AND CYTOCHEMISTRYThe relationship of the probe to the targetCytochemical localization of cell componentsCytochemical localization of enzymesCytochemical fluorescence microscopyFluorescent dyes for specific applicationsIntrinsic fluorescenceBackground fluorescence and photobleaching12. LOCALIZATION OF MOLECULAR TARGETS IN TISSUESImmunolocalizationExample protocols for immunolocalizationTissue printing to detect proteins and RNA in plant tissueIn situ hybridizationAutoradiography--Detecting incorporated radioactive elements in tissue sectionsWhole-mount in situ hybridizationTyramide signal amplificationTUNEL Assay for detecting DNA degradation and programmed cell deathFluorescence in situ hybridizationAPPENDICESI. ToxicsII. BuffersIII. General Information and Common CalculationsIV. The Mercury Arc Lamp in Fluorescence MicroscopyV. Manufacturers and VendorsVI. OpticsBibliographyIndex
SynopsisPlant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory., Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice ofmodern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditionalparaffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both textand diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to themicroscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory., This text provides in-depth coverage of the field of microtechnique. The traditional paraffin method is covered in explicit step-by-step protocols, with theoretical background included to give students the tools required to design their own experiments and interpret existing results. In addition, the theory and practice of modern applications, such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in-situ hybridization, are documented thoroughly. Preceding the first chapter are ten single-page Quick Start protocols. Patterned after computer software manuals, these abbreviated instructions provide the experienced student with the fundamental steps to complete the ten microtechnique protocols most frequently used today. Appendices on laboratory practice (chemical toxicities, common calculations and buffer tables) are provided., This book fills the need for a current textbook and reference of methods in plant microtechnique. It includes traditional methods of preparing, sectioning, and staining materials, fluorescence cytochemistry and immunolocalization, molecular techniques of in situ hydridization, plastic techniques for light microscopy, and video and digital microscopy.
LC Classification NumberQK673.R89 1999
