MOMENTAN AUSVERKAUFT
Über dieses Produkt
Product Identifiers
PublisherElsevier Science & Technology
ISBN-100123738768
ISBN-139780123738769
eBay Product ID (ePID)65908760
Product Key Features
Number of Pages416 Pages
Publication NameStem Cell Culture
LanguageEnglish
SubjectLife Sciences / Cell Biology, Microbiology
Publication Year2008
TypeTextbook
AuthorJennie P. Mather
Subject AreaScience, Medical
SeriesMethods in Cell Biology Ser.
FormatHardcover
Dimensions
Item Height0.4 in
Item Weight36.1 Oz
Item Length9.3 in
Item Width7.5 in
Additional Product Features
Edition Number86
Intended AudienceScholarly & Professional
Dewey Edition22
Series Volume NumberVolume 86
IllustratedYes
Volume NumberVolume 86
Dewey Decimal616.02774
Table Of ContentContributors to Volume 86 Preface Biography Derivation of Human Embryonic Stem Cells in Standard and Chemically Defined Conditions I Introduction II Methods III Discussion IV Materials Autogeneic Feeders for the Culture of Undifferentiated Human Embryonic Stem Cells in Feeder and Feeder'eFree Conditions I Introduction II Methods and Materials III Results IV Discussion and Summary Microporous Membrane Growth Substrates for Embryonic Stem Cell Culture and Differentiation I Introduction II Rationale III Methods IV Summary Culture of Rodent Spermatogonial Stem Cells, Male Germline Stem Cells of the Postnatal Animal I Introduction II Rationale III Methods IV Materials V Discussion Characterization of Human Amniotic Fluid Stem Cells and Their Pluripotential Capability I Introduction II Amniotic Fluid Cell Composition and Stem Cells III In Vitro Potential of Amniotic Fluid Stem Cells IV Ex Vivo and In Vivo Application of Amniotic Fluid Stem Cells V Discussion Method to Isolate Mesenchymal'eLike Cells from Wharton''s Jelly of Umbilical Cord I Introduction II Rationale III Methods IV Materials V UCMSCs and Culture Characteristics VI Discussion Isolation, Characterization, and Differentiation of Human Umbilical Cord Perivascular Cells (HUCPVCs) I Introduction II Methods III Concluding Remarks Hepatic Stem Cells and Hepatoblasts: Identification, Isolation, and Ex Vivo Maintenance I Introduction II Epithelial'e"Mesenchymal Relationship III Stem Cells and Lineage Biology IV Liver Processing V Fractionation of Liver Cell Subpopulations VI Feeder Cells VII Extracellular Matrix VIII Culture Media IX Monolayer Cultures of Cells X Three'eDimensional Systems Acknowledgments Appendix 1: Nomenclature, Glossary, and Abbreviations Appendix 2: Details on the Preparation of Buffers/Reagents Appendix 3: Commercial Sources of Reagents Appendix 4: Sources of Primary and Secondary Antibodies for Immunoselection and for Characterization of the Cells. (See Below for Suppliers and the Location of the Suppliers) Culture of Pluripotent Neural Epithelial Progenitor Cells from E9 Rat Embryo I Introduction II Rationale III Methods IV Materials V Discussion VI Summary Primary and Multipassage Culture of Human Fetal Kidney Epithelial Progenitor Cells I Introduction II Rationale III Methods IV Materials V Discussion VI Summary Isolation of Human Mesenchymal Stem Cells from Bone and Adipose Tissue I Introduction II Isolation of hMSCs from Trabecular Bone III Isolation of hMSCs from Adipose Tissue IV Growth, Subculture, and Cryopreservation of Undifferentiated hMSCs V Surface Marker Characterization of hMSCs VI hMSC Differentiation Assays for Osteogenic, Adipogenic, and Chondrogenic Pathways VII Summary Culture of Mesenchymal Stem/Progenitor Cells in Adhesion'eIndependent Conditions I Introduction II Suspension Culture of Bone Marrow'eDerived MPCs III Analysis of Suspension Culture IV Mechanism of MPC Expansion in Suspension V Discussion Purification and Long'eTerm Culture of Multipotent Progenitor Cells Affiliated with the Walls of Human Blood Vessels: Myoendothelial Cells and Pericytes I Introduction II Materials and Methods: Cell Analysis and Purification by Flow Cytometry III Materials and Methods: Long'eTerm Culture of Human Blood Vessel'eAssociated Progenitor Cells IV Materials and Methods: Proliferation Kinetics of Human Myoendothelial Cells and Pericytes V Materials and Methods: Genotypic and Phenotypic Analyses of Long'eTerm Cultured Cells VI Conclusion Isolation and Culture of Colon Cancer Stem Cells I Introduction II Identification of CSCs in Colon Cancer Tissue III Isolation and Propagation of Colon CSCs IV Discussion Isolation and Establishment of Human Tumor Stem Cells I Introduction II Rationale III Methods IV Results V Discussion VI Materials Stem Cells from Cartilaginous and Bony Fish I Introduction to Models and Uses II Embryonal Stem Cells III Tissue'eSpecific Stem Cells IV Outlook and Future Contributions Index Volume in Series
SynopsisThe purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture. Describes techniques in stem cell research Delineates critical steps and potential pitfalls for each method Covers specific procedures in dealing with Human Embryonic Stem Cells, Interest in the therapeutic value of embryonic, fetal and adult stem cell types is rapidly expanding throughout the scientific community. The first half of this century should see an explosion of therapeutic applications of stem cells which will grow from the cells and techniques described in this book. Stem Cell Culture provides a comprehensive resource for researchers in the fields of embryonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentiation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Chapters focus on the practical do's and don'ts of isolating and culturing these cell types, and use illustrative data or diagrams that allow the reader to confidently apply techniques and make this a standard reference. *Compares and contrasts a broad range of species and stem cell types. *Describes techniques in stem cell research, and goes beyond just protocols by presenting methods within perspective of recent literature reviews. *Delineates critical steps and potential pitfalls for each method, provided by at-the-bench authors for expert information. *Covers specific procedures in dealing with Human Embryonic Stem Cells, but also emphasizes other sources of potentially therapeutic stem cells such as amniotic fluid, adult muscle and adipose tissue, and umbilical cord., The purpose of Stem Cell Culture is to provide a comprehensive resource for researchers in the fields of embyonic, fetal and adult stem cell biology to find methods for the purification, culture, and differentitation of these cell types, with the main emphasis on the maintenance of the stem cell phenotype in vitro. This volume will be the first to broadly cover multiple types of stem cell culture from different ages, organs and species. Authors will focus on the practical do's and don'ts of isolating and culturing these cell types, and feel free to use illustrative data or diagrams whereever this improves the comprehension of the reader. This should allow the reader to compare and contrast techniques and make this a standard reference for those in the field, or desiring to start stem cell culture.
LC Classification NumberQH588.S83
