Edition DescriptionRevised edition
Table Of ContentGetting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non-Ionic Samples: Reversed- and Normal-Phase HPLC. Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC. Gradient Elution. Systematic Approach to the Reversed-Phase Separation of Regular Samples. Computer-Assisted Method Development. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds. Chiral Separations. Preparative HPLC Separation. Quantitation (Including Trace Analysis). Completing the Method: Validation and Transfer. Appendices. Index.
SynopsisHigh Performance Liquid Chromatography (HPLC) is a common method for analyzing substances and is used by researchers to measure the amount of caffeine in coffee, for example, or the level of penicillin in a blood sample. The process involves separating a substance into its individual components and then examining the result., This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations., This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.
LC Classification NumberQP519.9.H53S69 1997