Mature seeds of groundnut varieties (Golden, Bari-2007, Chico, P.W, No.334, 2KCG020, 2KCG017, 96CG005, 01CG005 and 02CG005) were procured from Barani Agricultural Research Institute (BARI) Chakwal, Pakistan and were evaluated for chemical and fatty acids profile. BARI-2000 gave maximum (100-seed) weight 60.26g and width/length ratio was found to be highest in Chico (0.66) seeds. 01CG009 gave highest crude protein content 31.69%. The fat content of groundnut varieties 02CG005, 96CG005, P.W, 2KCG017, Chico, 01CG009, 2KCG020, No. 334, BARI-2000, Golden was 49.9, 42.65, 44, 46.65, 41.38, 45.31, 50.39, 51.82 and 43.3% respectively. The iron (Fe) content was found to be maximum (3.68 mg/100g) in groundnut variety Chico. The results of the present study indicated that groundnut varieties differ in their oil and protein contents. Most important varieties in this respect were Bari-2000 and 01CG009 varieties which should be recommended for commercial utilization.