Alkaline proteases are robust enzymes with considerable industrial potential in detergents, leather processing, silver recovery, medical purposes, food processing, feeds, and chemical industries, as well as waste treatment. These enzymes contribute to the development of high value-added applications or products by using enzyme-aided partial digestion. Actinomycetes play an important role in the decomposition of organic matter in soil. They are the producers of most of the bioactive metabolites and industrially important enzymes. They include numerous potentially useful compounds providing the widest range and most promising array of pharmacologically and agriculturally active compounds. When compared to other microbial products as, the activity profile of actinomycete products is very broad.Enzymes produced from actinomycetes seem to be very promising as immobilized preparations for use in routine clinical diagnostic tests. Hence in the present work alkaline protease from novel Streptomyces was isolated and characterized.