Abscisic acid (ABA) is a plant growth hormone that plays a key role in many physiological and developmental processes in plant cells. The present investigation examines differential gene expression in Vicia villosa under ABA treatment using differential display technique, in an attempt to identify stress genes whose expression is regulated by ABA and define their function. The results of the database sequence alignment identified 7 fragment sequences: At NADH dehydrogenase; Mt zinc finger CCHC type; Mt, Phaseolus vulgaris, Glycine max NBS-LRR plant resistance, Psychrobacter sp. arginine acetyl transferase, Clostridium acetobutylicum phosphoenolpyruvate synthase, Arabidopsis thaliana ATP binding/ uracil phosphoribosyl transferase and Arabidopsis thaliana heat shock protein binding. Results of this work are a first step towards understanding the molecular mechanism of ABA and the elucidation of the biochemical changes- at a molecular level- that occur in Vicia villosa plant in response to environmental stress. The identified stress genes could be ultimately used to improve the stress (abiotic and/or biotic) tolerance of economic crops by gene manipulation.
Ahmed Bahieldin, Ahmed Salem, Rania M. I. Abou Ali