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- KurzbeschreibungTissue engineering is an interdisciplinary field <br>which focuses on scaffold design to aid in tissue <br>replacement. One major difficulty involves the lack <br>of vasculature within these scaffolds. This leads <br>to replacement failure. We take the approach to <br>fabricate a scaffold that directs endothelial cell <br>(EC) migration to form planar capillary networks. In <br>vivo, a combination of topological, mechanical and <br>chemical cues dictates migration. Here, scaffold <br>composition, surface chemistry and shear stress were <br>used to direct migration. Experiments were designed <br>to optimize electrospun scaffolds for ECs. Results <br>indicated that ECs prefer to grow along fibers with <br>a 1-5 micron diameter. A novel bioassay chamber was <br>designed to optimize EC growth from an autologous <br>donor source. Murine aortas were dissected, <br>cannulated and perfused at low flow rates. Cell <br>density was unrelated to flow rate, but viability <br>was enhanced with higher flow. Long term culture <br>increased cell viability without affecting density. <br>This work should help elucidate a new method to <br>engineer blood vessels and should be useful for <br>anyone interested in optimizing biomaterial <br>scaffolds for use with ECs.
- AutorDavid Rubenstein
- Seiten176 Seiten
- Gewicht278 g
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